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Galactone-γ-lactone-dependent ascorbate biosynthesis alters wheat kernel maturation.

A Paradiso1, M C de Pinto, V Locato

  • 1Department of Biology, University of Bari, Bari, Italy.

Plant Biology (Stuttgart, Germany)
|February 4, 2012
PubMed
Summary
This summary is machine-generated.

Ascorbate (ASC) is crucial for kernel maturation. Enhancing ASC biosynthesis delays its decrease, positively impacting programmed cell death, dehydration, and improving kernel filling.

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Area of Science:

  • Plant Physiology
  • Biochemistry
  • Agricultural Science

Background:

  • Kernel development involves complex events like ascorbate (ASC) metabolism changes, protein storage, programmed cell death (PCD), and dehydration.
  • The metabolic interconnections between these kernel maturation events are not fully understood.

Purpose of the Study:

  • To investigate the metabolic relationship between ASC metabolism, PCD, kernel filling, and dehydration during kernel maturation.
  • To determine the role of ASC biosynthesis in regulating these developmental processes.

Main Methods:

  • Analysis of ASC metabolism, PCD, kernel filling, and dehydration over three years.
  • Comparison between plants under normal conditions and those with modified ASC synthesis (fed L-galactone-γ-lactone, GL).
  • Measurement of ascorbate peroxidase (APX) activity.

Main Results:

  • A decrease in ASC content occurs during normal kernel maturation.
  • Enhanced ASC biosynthesis (via GL) delayed ASC decrease, APX activity enhancement, PCD, and dehydration.
  • Delayed ASC decrease correlated with improved kernel filling.

Conclusions:

  • Ascorbate plays a pivotal role in orchestrating kernel maturation events.
  • Ascorbate-dependent redox regulation is relevant for kernel productivity.
  • Manipulating ASC biosynthesis offers a potential strategy to enhance crop yield.