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Related Experiment Video

Updated: May 25, 2026

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

Nanoscopy in a living mouse brain.

Sebastian Berning1, Katrin I Willig, Heinz Steffens

  • 1Department of NanoBiophotonics, Max Planck Institute (MPI) for Biophysical Chemistry, Göttingen, Germany.

Science (New York, N.Y.)
|February 4, 2012
PubMed
Summary
This summary is machine-generated.

Superresolution optical microscopy was achieved in a living animal. Stimulated emission depletion (STED) fluorescence nanoscopy visualized mouse cerebral cortex neurons with nanoscale resolution, enabling observation of dendritic spines over time.

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Related Experiment Videos

Last Updated: May 25, 2026

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
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Published on: October 8, 2013

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Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases
07:47

Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases

Published on: December 6, 2016

Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Microscopy

Background:

  • Understanding neuronal structure and dynamics is crucial in neuroscience.
  • Existing microscopy techniques have limitations in resolving fine cellular structures in vivo.
  • Superresolution microscopy offers potential for higher resolution imaging.

Purpose of the Study:

  • To demonstrate superresolution optical microscopy in a living higher animal.
  • To achieve nanoscale resolution imaging of neurons in the mouse cerebral cortex.
  • To enable long-term observation of dendritic spines and their changes.

Main Methods:

  • Utilized Stimulated Emission Depletion (STED) fluorescence nanoscopy.
  • Applied STED nanoscopy to image the cerebral cortex of a living mouse.
  • Performed time-lapse imaging to observe cellular dynamics.

Main Results:

  • Achieved superresolution imaging with <70-nanometer resolution in a living animal.
  • Successfully visualized neurons in the mouse cerebral cortex.
  • Observed dendritic spines and their subtle changes at relevant scales over extended periods.

Conclusions:

  • STED fluorescence nanoscopy is effective for in vivo superresolution imaging in higher animals.
  • This technique allows for detailed observation of neuronal structures like dendritic spines.
  • Enables studying dynamic cellular processes in the brain at the nanoscale.