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Updated: Aug 11, 2026
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
Published on: March 25, 2014
An immunoassay for the quantitation of cell membrane MHC class II antigen
1Department of Veterinary Medicine, University of Bristol, Langford, U.K.
Abstract:
A method is described for the quantitative assay of cellular MHC class II proteins. The assay has been developed for special use with epithelial cells isolated from the intestine, but has been successfully used with other cell types. It comprises a competitive immunoassay of anti-class II activity in cell lysates, using IgG-coated Sephacryl S300 as the solid phase and is sensitive over the range 1 ng-1 microgram.
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