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Updated: May 25, 2026

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
Published on: October 15, 2016
Expression of high-affinity human antibody fragments in bacteria
Romain Rouet1, David Lowe, Kip Dudgeon
1Garvan Institute of Medical Research, Darlinghurst, Sydney, New South Wales, Australia.
Abstract:
Here we describe protocols for the expression of human antibody fragments in Escherichia coli. Antigen-specific clones are identified by soluble fragment ELISA and concentrated by periplasmic preparation. They are then further purified by affinity chromatography. This article provides an overview of expression and purification strategies for human antibody fragments, as well as detailed protocols for the identification of high-affinity binders and for affinity maturation.
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