Related Experiment Video
Updated: May 25, 2026
Operation Procedure and Precautions of Lingnan Fire-Needle Therapy in Osteoporosis Model Rats
Published on: April 26, 2024
Protective effect of amlodipine on rat bone tissue after orchidectomy
Iveta Gradosova1, Helena Zivna, Vladimir Palicka
1Institute of Clinical Biochemistry and Diagnostics, Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Kralove, Kralove, Czech Republic. gradosovai@ lfhk.cuni.cz
Aim:
Our study aimed to investigate the effect of amlodipine on bone metabolism in orchidectomized rats.
Methods:
Eight-week-old rats were divided into three groups. The sham-operated control group (SHAM) and the control group after orchidectomy (ORX) received the standard laboratory diet (SLD). The experimental group after orchidectomy (ORX+AML) received SLD enriched with amlodipine for 12 weeks. Bone marker concentrations in serum of PINP, OPG and IGF-1, and the levels of CTX-I, BAP and BMP-2 in a bone homogenate were measured using enzyme-linked immunosorbent assay. Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry. The femurs were used for biomechanical testing.
Results:
Bone markers (CTX-I, BAP, BMP-2) in ORX were higher versus SHAM. In ORX+AML there was a decrease in PINP, CTX-I, BAP, BMP-2 and OPG versus ORX. IGF-1 was decreased in ORX versus SHAM. In ORX+AML it was increased versus ORX. In ORX, a decrease was demonstrated versus SHAM in BMD of the whole body, in the lumbar vertebrae and in both femurs. In ORX+AML there was an increase in BMD of the whole body versus ORX. Three-point bending test revealed a decrease in maximal load values in ORX versus SHAM. After amlodipine administration there was an increase in the left femur versus ORX.
Conclusions:
Amlodipine is capable of mitigating the negative effects of orchidectomy and could be a good prevention of osteoporosis.
