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Updated: May 25, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
Multidimensional Raman spectroscopic signature of sweat and its potential application to forensic body fluid
Vitali Sikirzhytski1, Aliaksandra Sikirzhytskaya, Igor K Lednev
1Department of Chemistry, University at Albany, SUNY, Albany, NY 12222, United States.
Abstract:
This proof-of-concept study demonstrated the potential of Raman microspectroscopy for nondestructive identification of traces of sweat for forensic purposes. Advanced statistical analysis of Raman spectra revealed that dry sweat was intrinsically heterogeneous, and its biochemical composition varies significantly with the donor. As a result, no single Raman spectrum could adequately represent sweat traces. Instead, a multidimensional spectroscopic signature of sweat was built that allowed for the presentation of any single experimental spectrum as a linear combination of two fluorescent backgrounds and three Raman spectral components dominated by the contribution from lactate, lactic acid, urea and single amino acids.
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