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Updated: Aug 21, 2026
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In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
Synthesis and characterization of a new substrate of Photinus pyralis luciferase: 4-methyl-D-luciferin
C Farace1, B Blanchot, D Champiat
1CEMAGREF, Laboratoire de Biochimie, Lyon, France.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|July 1, 1990
Abstract:
A derivative of luciferin, 4-methyl-D-luciferin, was synthesized in low yield, and characterized by its ultraviolet and fluorescence spectra. The spectra of luciferin and its derivative are very similar. 4-Methyl-D-luciferin is a substrate of Photinus pyralis luciferase, and it causes light emission in luminometric assays.
