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Updated: May 25, 2026

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
Published on: November 11, 2022
Degradation of actin nucleators affects cortical polarity of aged mouse oocytes
Shao-Chen Sun1, Wei-Wei Gao, Yong-Nan Xu
1Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, South Korea.
Objective:
To investigate the molecular mechanism of mouse oocyte polarity loss during aging.
Design:
Experimental study.
Setting:
Academic basic research laboratory.
Animal(S):
Mice.
Intervention(S):
Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine.
Main Outcome Measure(S):
Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription-polymerase chain reaction.
Result(S):
The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes.
Conclusion(S):
Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes.
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