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Updated: May 25, 2026

Autofluorescence Imaging to Evaluate Red Algae Physiology
Published on: February 17, 2023
Studying photoprotective processes in the green alga Chlorella pyrenoidosa using nonlinear laser fluorimetry
Victor V Fadeev1, Maxim Y Gorbunov, Timofey S Gostev
1Physics Department, M. V. Lomonosov Moscow State University, Leninskie Gory, 1, Block 2, GSP-1, 119991 Moscow, Russia.
Abstract:
We use an advanced fluorescence method of Nonlinear Laser Fluorimetry in combination with Fluorescence Induction and Relaxation technique to study the influence of excess-light conditions on the physiological state of the green alga Chlorella pyrenoidosa. We demonstrate that zeaxanthin-dependent non-photochemical quenching leads to a significant increase in the rate constant of singlet-singlet annihilation of chlorophyll a excited state, which suggests profound conformational changes in the light-harvesting complexes of photosystem II.
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