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Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
Published on: September 22, 2017
Quantitative discrimination of NPC cell lines using optical coherence tomography
Jianghua Li1, Changshui Chen, Bingling Chen
1School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China.
Abstract:
We tried to explore the intrinsic differences in the optical properties of the four representative NPC cell lines on the models of radiobiology and metastasis by OCT. The scattering coefficients and anisotropies were extracted by fitting the average a-scan attenuation curves based on the multiple scatter effect. The values of scattering coefficients and anisotropy factors were 5.21 ± 0.11, 5.30 ± 0.09, 5.92 ± 0.21, 6.97 ± 0.22, and 0.892 ± 0.009, 0.886 ± 0.006, 0.884 ± 0.009, 0.86 ± 0.01 for CNE1, CNE2, 5-8F and 6-10B pellets (p < 0.05, P = 0.07 for CNE1 and CNE2), respectively. The results showed that the radiobiology and metastasis cell's model could be distinguished obviously; which implied that the corresponding types of NPC tissue might be potentially differentiated by OCT.
Insights
Optical coherence tomography (OCT) revealed distinct optical properties in nasopharyngeal carcinoma (NPC) cell lines, differentiating radiobiology and metastasis models. This suggests OCT
Area of Science:
- Biomedical Optics
- Cancer Research
- Cell Biology
Background:
- Nasopharyngeal carcinoma (NPC) exhibits diverse biological behaviors.
- Understanding intrinsic optical differences in NPC cell lines is crucial for diagnosis.
- Current methods may not fully capture these cellular variations.
Purpose of the Study:
- To investigate the intrinsic optical property differences among four representative NPC cell lines.
- To correlate these optical properties with radiobiology and metastasis models.
- To assess the potential of Optical Coherence Tomography (OCT) for differentiating NPC tissues.
Main Methods:
- Utilized Optical Coherence Tomography (OCT) to analyze NPC cell line models.
- Extracted scattering coefficients and anisotropy factors from OCT data.
- Employed a multiple scatter effect model to fit a-scan attenuation curves.
Main Results:
- Significant differences in scattering coefficients and anisotropy factors were observed among CNE1, CNE2, 5-8F, and 6-10B cell lines.
- Specific values for scattering coefficients ranged from 5.21 to 6.97, and anisotropy factors from 0.86 to 0.892.
- OCT clearly distinguished between the radiobiology and metastasis NPC cell models.
Conclusions:
- Intrinsic optical properties vary significantly between different NPC cell lines.
- OCT can effectively differentiate NPC cell models based on radiobiology and metastasis.
- OCT shows potential for in vivo differentiation of NPC tissue types.
