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Embryonic neural cell adhesion molecule in cerebrospinal fluid of younger children: age-dependent decrease during the

C Weisgerber1, M Husmann, M Frosch

  • 1Institute of Medical Microbiology, Hannover; F.R.G.

Journal of Neurochemistry
|December 1, 1990
PubMed
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Poly-alpha-2,8-N-acetylneuraminic acid (poly-alpha-2,8-NeuAc), found in neural tissue and linked to NCAM, was quantified in children's CSF. Levels decreased significantly over the first year of life.

Area of Science:

  • Neuroscience
  • Immunology
  • Glycobiology

Background:

  • Poly-alpha-2,8-N-acetylneuraminic acid (poly-alpha-2,8-NeuAc) is a developmentally regulated carbohydrate found on neural cell adhesion molecule (NCAM) in higher animals.
  • Bacterial poly-alpha-2,8-NeuAc is a key virulence factor for pathogens like Neisseria meningitidis group B and E. coli K1, causing bacterial meningitis.

Purpose of the Study:

  • To develop and apply a quantitative assay for poly-alpha-2,8-NeuAc in cerebrospinal fluid (CSF) from children.
  • To determine the levels and binding form of poly-alpha-2,8-NeuAc in pediatric CSF.

Main Methods:

  • Development of a quantitative enzyme-linked immunoassay using monoclonal antibody (MAb) 735, specific for poly-alpha-2,8-NeuAc.
  • Application of the immunoassay to CSF specimens from children.

Related Experiment Videos

  • Characterization of poly-alpha-2,8-NeuAc binding partners using immunoprecipitation, immunoblotting, affinity enrichment, and SDS-PAGE.

    • Main Results:

    • Poly-alpha-2,8-NeuAc was detected in pediatric CSF at concentrations ranging from 20-0.2 micrograms/ml.
    • CSF poly-alpha-2,8-NeuAc levels showed a significant decrease from day 1 to day 300 of life.
    • Analysis confirmed that poly-alpha-2,8-NeuAc in CSF is bound to human NCAM, likely the NCAM-120 isoform.

    Conclusions:

    • The study successfully quantified poly-alpha-2,8-NeuAc in pediatric CSF, revealing age-dependent changes.
    • The findings indicate that human NCAM, particularly NCAM-120, serves as a carrier for poly-alpha-2,8-NeuAc in the CSF.
    • This research provides insights into the presence and behavior of this developmentally important glycan in the central nervous system of infants.