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Updated: May 25, 2026
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid
Simona Biagi1, Silvia Ghimenti, Massimo Onor
1National Research Council of Italy, C.N.R., Istituto per i Processi Chimico-Fisici-IPCF- UOS Pisa, Area di Ricerca, Via G. Moruzzi 1, 56124, Pisa, Italy.
Abstract:
The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high-performance liquid chromatography (RP-HPLC) with UV detection at 220 nm. The calibration curves were linear in the investigated range 0.3 - 350 mm of lactate, 0.003- 1 mm of pyruvate. The sensitivity was good with a limit of detection of 0.03 mm for lactate and 0.001 mm for pyruvate. Recoveries evaluated for the entire procedure were 102 ± 0.1 and 96 ± 0.1 for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise.
