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Updated: May 25, 2026
09:18
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
Published on: April 21, 2022
Advancing cell biology through proteomics in space and time (PROSPECTS)
Angus I Lamond1, Mathias Uhlen, Stevan Horning
1Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH United Kingdom. a.i.lamond@dundee.ac.uk
Molecular & Cellular Proteomics : MCP
|February 8, 2012
Summary
Proteomics advances enable sensitive, quantitative protein analysis for cell biology. The PROSPECTS Network developed new methods for functional proteome analysis, driving a new generation of proteomics tools.
Area of Science:
- Proteomics
- Mass Spectrometry
- Cell Biology
Background:
- Proteomics has rapidly advanced due to improvements in mass spectrometry and data analysis.
- It offers sensitive and quantitative methods for studying protein structure and dynamics.
- Understanding cellular mechanisms in human cells and model organisms is a key goal.
Purpose of the Study:
- To present progress from the EU-funded Proteomics Specification in Time and Space (PROSPECTS) Network.
- To highlight the development of new methods and applications for functional analysis of cellular proteins.
- To showcase advancements in proteomics for cell biology and molecular medicine.
Main Methods:
- Improvements in Orbitrap mass spectrometer resolution and sensitivity.
- Systematic protein detection using antibody collections.
- Development of absolute and relative protein quantification methods.
Main Results:
- Demonstrated quantitative measurements of cell proteomes.
- Enabled study of dynamic proteome responses to perturbations.
- Showcased advancements in high-sensitivity protein identification.
Conclusions:
- Proteomics is evolving beyond identification to dynamic characterization.
- A new "third generation" proteomics strategy is emerging.
- This strategy provides essential tools for cell biology and molecular medicine.
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