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Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
Published on: July 15, 2018
Method for protein subfractionation of cardiovascular tissues before DIGE analysis
Athanasios Didangelos1, Xiaoke Yin, Manuel Mayr
1Cardiovascular Division, King's College London, London, UK.
Methods in Molecular Biology (Clifton, N.J.)
|February 8, 2012
Summary
Difference gel electrophoresis (DIGE) is challenging for cardiovascular tissue analysis due to protein contamination. This study introduces a new biochemical subfractionation method to improve DIGE results for heart tissues.
Area of Science:
- Proteomics
- Cardiovascular Research
- Biochemistry
Background:
- Difference gel electrophoresis (DIGE) is a common technique in cardiovascular research.
- Analysis of cardiovascular tissues using DIGE is often hindered by abundant plasma proteins and extracellular matrix components, limiting dynamic range.
Purpose of the Study:
- To develop and present a novel methodology for the biochemical subfractionation of cardiovascular tissues.
- To overcome the limitations of DIGE analysis in complex cardiovascular samples.
Main Methods:
- Biochemical subfractionation of cardiovascular tissues.
- Subsequent analysis using Difference gel electrophoresis (DIGE).
Main Results:
- The described methodology enables effective biochemical subfractionation of cardiovascular tissues.
- This pre-analysis step enhances the feasibility of DIGE for complex cardiovascular samples.
Conclusions:
- The novel subfractionation technique improves the analysis of cardiovascular tissues by DIGE.
- This method addresses dynamic range limitations caused by protein contaminants and abundant extracellular matrix components.
