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Related Concept Videos

Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Target-induced SOFA-HDV ribozyme.

Michel V Lévesque1, Jean-Pierre Perreault

  • 1Département de Biochimie, Université de Sherbrooke, Sherbrooke, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|February 9, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method for designing highly specific Hepatitis Delta Virus (HDV) ribozymes. These engineered ribozymes offer improved tools for functional genomics and gene therapy applications.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Gene Regulation

Background:

  • Ribozymes are RNA molecules with catalytic activity, capable of cleaving RNA substrates.
  • The Hepatitis Delta Virus (HDV) ribozyme is unique as it originates from human cells, enabling function within human protein environments.
  • HDV ribozymes exhibit remarkable stability in human cells, presenting advantages for biotechnological applications.

Purpose of the Study:

  • To describe an efficient, step-by-step approach for designing highly specific SOFA-HDV ribozymes.
  • To present a method minimizing time and effort in ribozyme design.
  • To provide a refined tool for functional genomics and gene therapy.

Main Methods:

  • Engineering of cis-acting ribozymes into trans-acting RNA-cleaving molecules.
  • Development of a specific on/off adaptor (SOFA) to enhance ribozyme specificity.
  • Application of SOFA-HDV ribozyme systems in both prokaryotic and eukaryotic cells.

Main Results:

  • Creation of a new generation of HDV ribozymes with significantly improved specificity.
  • Demonstration of SOFA-HDV ribozyme efficacy in gene inactivation systems.
  • Establishment of a streamlined protocol for designing potent and specific ribozymes.

Conclusions:

  • The developed method facilitates the efficient design of highly specific SOFA-HDV ribozymes.
  • These engineered ribozymes represent a valuable tool for advancing functional genomics and gene therapy.
  • The SOFA-HDV system offers a promising platform for precise gene manipulation in diverse cellular contexts.