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Related Experiment Video

Updated: May 25, 2026

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
08:12

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Published on: March 20, 2013

A large-scale (19)F MRI-based cell migration assay to optimize cell therapy.

F Bonetto1, M Srinivas, B Weigelin

  • 1Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.

NMR in Biomedicine
|February 9, 2012
PubMed
Summary

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Developing novel cell therapies requires understanding cell migration. This study introduces a new assay to quantify cell migration, revealing that cell density significantly impacts migration efficiency, crucial for therapeutic success.

Area of Science:

  • Cellular Biology
  • Immunology
  • Biomedical Engineering

Background:

  • Adoptive cell therapy necessitates efficient cell delivery and migration to target organs for therapeutic efficacy.
  • Dendritic cells (DCs) must migrate to lymph nodes for effective antigen presentation and immune response induction.
  • In vivo studies of cell migration are challenging, hindering optimization of cell delivery methods.

Purpose of the Study:

  • To develop and validate a novel in vitro assay for quantitative assessment of cell migration.
  • To optimize cell delivery strategies for therapeutic applications, focusing on dendritic cells.
  • To investigate the impact of cell density on cell migration in a 3D environment.

Main Methods:

  • Development of a novel fluorine-19 (19F) Magnetic Resonance (MR)-based assay for large-scale, quantitative cell migration measurement.

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  • Utilizing a three-dimensional collagen scaffold to mimic in vivo tissue conditions.
  • Labeling primary human DCs with a clinically applicable 19F label and imaging cell migration along a chemokine gradient.

    • Main Results:

    • The assay successfully quantified cell migration in a 3D collagen scaffold under chemokine-driven conditions.
    • Significant cell migration (up to 3%) was observed with fewer than one million cells.
    • No migration was detected when using larger cell numbers, indicating a density-dependent effect.

    Conclusions:

    • Cell density in delivery boluses critically influences migration efficiency, potentially explaining limited migration in clinical settings.
    • The developed 19F MR-based assay provides a powerful tool for optimizing cell delivery and migration in vitro.
    • This research offers insights into improving cell-based therapies by addressing cell delivery and migration challenges.