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Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Prokaryotes

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...

Replication in Prokaryotes

Replication in Prokaryotes

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Related Experiment Video

Updated: May 25, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Replication timing: the early bird catches the worm.

Max E Douglas¹, John F X Diffley

¹Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, UK.

Current Biology : CB

|February 11, 2012

Summary

Eukaryotic DNA replication origins fire at distinct times during S phase. Origin firing time correlates with their capacity to recruit essential, but limited, replication initiation factors.

Area of Science:

Molecular Biology
Cell Cycle Regulation
Genetics

Background:

Eukaryotic DNA replication is a complex process.
Replication origins are specific DNA sequences where replication initiates.
The timing of origin activation during S phase is crucial for genome stability.

Purpose of the Study:

To investigate the relationship between replication origin firing time and the recruitment of limiting replication initiation factors.
To understand the molecular mechanisms governing the temporal activation of replication origins.

Main Methods:

Analysis of replication origin activity.
Quantification of replication initiation factor recruitment.
Cellular assays to assess S phase progression.

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

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Last Updated: May 25, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

Main Results:

Replication origins that fire earlier in S phase exhibit a higher capacity for recruiting rate-limiting initiation factors.
The availability of specific initiation factors directly influences the temporal firing of replication origins.
A correlation was established between origin firing time and the abundance of limiting initiation factors at the origin site.

Conclusions:

The temporal control of DNA replication origin firing in eukaryotes is regulated by the differential recruitment of limiting replication initiation factors.
This mechanism ensures efficient and orderly genome duplication during S phase.