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Related Experiment Video

Updated: May 25, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Single cell electroporation using microfluidic devices.

Séverine Le Gac1, Albert van den Berg

  • 1BIOS the Lab-on-a-Chip Group, MESA+ Institute for Nanotechnology, University of Twente, Enschede, The Netherlands. S.LeGac@ewi.utwente.nl

Methods in Molecular Biology (Clifton, N.J.)
|February 11, 2012
PubMed
Summary

This study introduces a microfluidic device for single-cell electroporation, enabling precise control over cell membrane permeability and material delivery at the individual cell level for various biological applications.

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Last Updated: May 25, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
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Published on: January 7, 2022

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Published on: August 7, 2014

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Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Electroporation is a method to enhance cell membrane permeability for introducing foreign substances.
  • Traditional bulk electroporation operates at the milliliter scale, processing millions of cells.
  • Miniaturization to single-cell electroporation offers enhanced precision and control.

Purpose of the Study:

  • To develop and detail a microfluidic device for single-cell electroporation.
  • To demonstrate the capability of the device for precise material delivery into individual cells.
  • To showcase applications including dye loading, plasmid transfection, and signaling pathway analysis.

Main Methods:

  • Development of a microfluidic chip with an array of independent electroporation sites.
  • Cell trapping within micrometer-sized structures for in situ electric field exposure.
  • Detailed protocol including cell preparation, trapping, electroporation, and on-chip detection.

Main Results:

  • Successful demonstration of dye transport across the plasma membrane of single cells.
  • Efficient transfection of single cells with GFP-encoding plasmids.
  • Analysis of the ERK1 signaling pathway in single cells post-transfection using GFP-ERK1.

Conclusions:

  • The microfluidic device enables precise, customized single-cell electroporation.
  • The platform integrates multiple biological processes (poration, culture, imaging) on a single chip at the single-cell level.
  • This approach significantly advances research capabilities in cell biology and biotechnology.