Ultrasonic manipulation of single cells
1Department of Biomedical and X-Ray Physics, Royal Institute of Technology, Stockholm, Sweden. martin.wiklund@biox.kth.se
Methods in Molecular Biology (Clifton, N.J.)
|February 11, 2012
Summary
Ultrasonic manipulation offers precise control for trapping and separating cells at the single-cell level. This technique combines ultrasound with microfluidics for advanced cell handling and interaction studies.
Area of Science:
- Acoustic manipulation
- Microfluidics
- Cell biology
Background:
- Ultrasonic manipulation is a powerful tool for cell trapping, aggregation, and separation.
- Acoustophoresis, a key application, excels with high-density cell suspensions.
- Microscale applications have significantly advanced in the last decade.
Purpose of the Study:
- To describe combining ultrasound with microfluidics and microplates for single-cell manipulation.
- To demonstrate methods for selecting, trapping, aggregating, and positioning individual cells.
- To discuss applications in immune cell interaction studies.
Main Methods:
- Utilizing multifrequency ultrasonic actuation for precise cell control.
- Integrating ultrasound with microfluidic devices and microplates.
- Developing methods for handling and manipulating cells at the single-cell level.
Main Results:
- Demonstrated successful selection, trapping, aggregation, and positioning of individual cells.
- Showcased the potential of ultrasound in microdevices for cell manipulation.
- Explored applications relevant to immune cell interaction studies.
Conclusions:
- Ultrasound combined with microfluidics enables precise single-cell manipulation.
- The technology offers versatile cell handling for research and potential applications.
- Further applications in studying cellular interactions, particularly immune cells, are promising.
