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Published on: April 18, 2016
The immune inhibitory complex CD200/CD200R is developmentally regulated in the mouse brain
Kalpana Shrivastava1, Pau Gonzalez, Laia Acarin
1Medical Histology, Institute of Neuroscience, Department of Cell Biology, Physiology, and Immunology, Universitat Autonoma Barcelona, Bellaterra 08193, Barcelona, Spain. kalpana.shrivastava@uab.cat
The Journal of Comparative Neurology
|February 11, 2012
Summary
The CD200/CD200R immune system regulates brain microglia. This study maps CD200 and CD200R expression during mouse development, revealing roles in microglial maturation and renewal.
Area of Science:
- Neuroimmunology
- Developmental Neuroscience
Background:
- The CD200/CD200R pathway is a key regulator of microglial function in the adult brain.
- Microglial maturation occurs during postnatal development, a process not fully understood in relation to CD200/CD200R signaling.
Purpose of the Study:
- To investigate the spatiotemporal expression of CD200 and CD200R in the developing and adult mouse brain.
- To elucidate the role of the CD200/CD200R system in microglial development and maturation.
Main Methods:
- Immunofluorescent labeling and Western blotting were employed to analyze CD200 and CD200R expression.
- Expression patterns were examined across various postnatal developmental stages and in adult C57/BL6 mice.
Main Results:
- CD200 expression peaked at postnatal days 5-7 and was found around neurons, in cortical layer I, and on astrocytes and endothelium.
- CD200R expressing cells, primarily microglia/macrophages, were abundant in early development, decreasing with age, and localized to meninges, ventricles, and perivascular spaces.
- Specific CD200R+ microglia were identified in the cingulum during early postnatal stages.
Conclusions:
- The CD200/CD200R system exhibits dynamic expression during brain development, interacting in distinct locations.
- These findings suggest a significant role for CD200/CD200R in microglial development, maturation, and renewal throughout postnatal life.
