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Related Experiment Video

Updated: May 25, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Novel RNA base pair with higher specificity using single selenium atom.

Huiyan Sun¹, Jia Sheng, Abdalla E A Hassan

¹Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA.

Nucleic Acids Research

|February 11, 2012

Summary

This summary is machine-generated.

Researchers synthesized selenium-modified uridine to improve RNA base pairing specificity. This modification enhances uridine-adenine pairing and discriminates against unwanted uridine-guanine wobble pairs, offering a chemical strategy for greater genetic information accuracy.

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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

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Published on: November 1, 2019

Area of Science:

Molecular Biology
Biochemistry
Chemical Biology

Background:

Nucleobase pairing is fundamental to genetic processes.
Uracil/guanine (U/G) wobble base pairs in RNA can reduce pairing specificity.
Non-coding RNAs frequently feature U/G wobble pairs.

Purpose of the Study:

To enhance the specificity of uridine/adenine (U/A) base pairing.
To discriminate against U/G wobble pairs in RNA.
To explore the effects of replacing oxygen with selenium at the 2-exo position of uridine.

Main Methods:

Synthesis of 2-selenium-substituted uridine (Se)U phosphoramidite and (Se)U-containing RNAs.
Biophysical characterization of (Se)U-RNAs.
Structural studies of RNA duplexes containing (Se)U.

Main Results:

Successful synthesis of (Se)U-RNAs and phosphoramidite.
Demonstrated enhanced specificity of the novel (Se)U/A base pair compared to the natural U/A pair.
Observed that the (Se)U/A pair maintains native U/A structure while effectively discriminating against U/G wobble pairs.

Conclusions:

Replacing the 2-exo oxygen of uridine with selenium is a viable chemical strategy to enhance base pairing specificity.
The (Se)U modification offers a method to improve the fidelity of genetic information storage and processing.
This atomic-level modification provides a novel approach to control RNA structure and function.