Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Particle sizing in milk by combined differential dynamic microscopy and cryo-FIB-SEM tomography.

Soft matter·2026

Same author

Collective motion in bacterial suspensions is scale-free.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Environmental dependence of colony morphologies in Labyrinthula species.

Journal of the Royal Society, Interface·2026

Same author

Heat-Induced Structural Changes in Lactoferrin for Enhanced Mucoadhesion.

ACS applied bio materials·2025

Same author

Impact of particle-size polydispersity on the quality of thin-film colloidal crystals.

Soft matter·2025

Same author

Electrostatics and viscosity are strongly linked in concentrated antibody solutions.

Proceedings of the National Academy of Sciences of the United States of America·2025

Search research articles

Related Experiment Video

Updated: May 25, 2026

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Published on: June 15, 2022

Differential dynamic microscopy for anisotropic colloidal dynamics.

Mathias Reufer¹, Vincent A Martinez, Peter Schurtenberger

¹SUPA and School of Physics Astronomy, The University of Edinburgh, Edinburgh, United Kingdom. mathias.reufer@ed.ac.uk

Langmuir : the ACS Journal of Surfaces and Colloids

|February 14, 2012

Summary

This summary is machine-generated.

Differential dynamic microscopy (DDM) now probes anisotropic particle dynamics. This method accurately measures diffusion parallel and perpendicular to fields, revealing orientational order.

More Related Videos

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Related Experiment Videos

Last Updated: May 25, 2026

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Published on: June 15, 2022

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Area of Science:

Colloid and Interface Science
Soft Matter Physics
Optical Microscopy Techniques

Background:

Differential dynamic microscopy (DDM) is a recent technique for analyzing spherical colloid diffusion.
DDM typically characterizes isotropic Brownian motion using white-light optical microscopy.
Characterizing anisotropic particle dynamics requires advanced analytical methods.

Purpose of the Study:

To extend Differential Dynamic Microscopy (DDM) for probing anisotropic colloidal dynamics.
To develop theoretical frameworks for DDM analysis of ordered phases and field-interacting particles.
To demonstrate the method's efficacy with anisotropic magnetic colloids.

Main Methods:

Theoretical development of DDM for anisotropic systems.
Measurement of q-dependent dynamics in multiple directions within the image plane.
Application to a dilute aqueous dispersion of hematite (magnetic) particles.

Main Results:

Successful application of DDM to anisotropic magnetic particles.
Measured diffusion coefficients parallel and perpendicular to the magnetic field align well with theoretical predictions.
Demonstrated extraction of the orientational order parameter S(2) from DDM data.

Conclusions:

DDM is a versatile technique applicable to anisotropic colloidal systems.
The developed method provides accurate measurements of anisotropic diffusion and orientational order.
This advancement expands the utility of DDM in soft matter research.