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Related Experiment Video

Updated: May 25, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

Studying lipids involved in the endosomal pathway.

Christin Bissig¹, Shem Johnson, Jean Gruenberg

¹Biochemistry Department, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva 4, Switzerland.

Methods in Cell Biology

|February 14, 2012

Summary

This chapter reviews how endosomal lipids regulate cell degradation pathways. It details methods to study lipid functions in endocytosis, focusing on lysobisphosphatidic acid.

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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Area of Science:

Cell Biology
Biochemistry

Background:

Endosomes in the degradation pathway are multivesicular and have distinct lipid compositions.
Protein-lipid and lipid-lipid interactions form functional membrane platforms regulating endosome function.
Lipid composition is crucial for endosome biogenesis and intracellular trafficking.

Purpose of the Study:

To review the roles of endosomal lipids in the degradation pathway.
To discuss techniques for studying lipid-based mechanisms in endocytosis.
To provide protocols for analyzing lipid functions in vivo and in vitro.

Main Methods:

Review of existing literature on endosomal lipid functions.
Discussion of various experimental techniques for lipid analysis.
Presentation of step-by-step protocols for in vivo and in vitro studies.

Main Results:

Endosomal lipids are key regulators of the degradation pathway.
Specific techniques enable monitoring of lipid distribution, levels, and dynamics.
The function of lysobisphosphatidic acid in late endosomes has been elucidated using these methods.

Conclusions:

Lipid-based mechanisms are critical for endosome function and biogenesis.
The described techniques are valuable for studying lipid roles in endocytosis.
Further research can leverage these methods to understand lipid-protein interactions in endosomal trafficking.