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Related Experiment Video

Updated: May 25, 2026

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Published on: August 21, 2017

Reconstituting multivesicular body biogenesis with purified components.

Thomas Wollert¹

¹Molecular Membrane and Organelle Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

Methods in Cell Biology

|February 14, 2012

Summary

Cell surface receptors are removed via endocytosis and sorted for degradation. The study uses in vitro systems to analyze the ESCRT machinery

Area of Science:

Cell Biology
Molecular Biology

Background:

Activated cell surface receptors are internalized via clathrin-mediated endocytosis.
Receptors destined for lysosomal degradation are sorted into intraluminal vesicles (ILVs) within endosomes.
This process, termed multivesicular body (MVB) biogenesis, involves the ESCRT machinery.

Purpose of the Study:

To investigate the molecular mechanisms of ESCRT-driven membrane remodeling during MVB biogenesis.
To utilize in vitro reconstitution systems for studying these complex cellular events.

Main Methods:

Generation of large and giant unilamellar liposomes (artificial membranes).
In vitro reconstitution of fluorescently labeled ESCRT proteins on liposomes.
Analysis of ESCRT-driven membrane remodeling reactions.

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Last Updated: May 25, 2026

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

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Published on: August 21, 2017

In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)

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Construction of Out-of-Equilibrium Metabolic Networks in Nano- and Micrometer-Sized Vesicles

Construction of Out-of-Equilibrium Metabolic Networks in Nano- and Micrometer-Sized Vesicles

Published on: April 12, 2024

Main Results:

Demonstration of ESCRT-mediated membrane budding and scission events on artificial membranes.
Visualization of protein complex assembly and membrane deformation.
Insights into the sequential action of ESCRT complexes.

Conclusions:

In vitro systems provide a powerful platform for dissecting the ESCRT machinery's function.
These systems allow for detailed mechanistic studies of membrane remodeling in MVB biogenesis.
Further research can refine these models to better understand receptor sorting and degradation.