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Related Experiment Video

Updated: May 25, 2026

A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva
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A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva

Published on: January 12, 2019

Evaluation of systemic microvascular endothelial function using laser speckle contrast imaging.

Ivan Cordovil1, Grazielle Huguenin, Glorimar Rosa

  • 1National Institute of Cardiology (INC), Rio de Janeiro, Brazil.

Microvascular Research
|February 14, 2012
PubMed
Summary

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Cardiometabolic disease impairs skin microvascular endothelial function, as shown by reduced vasodilator responses to acetylcholine (ACh) and post-occlusive reactive hyperemia (PORH). Laser speckle contrast imaging (LSCI) effectively evaluates this function.

Area of Science:

  • Cardiovascular Research
  • Vascular Biology
  • Biomedical Engineering

Background:

  • Cardiometabolic diseases are associated with endothelial dysfunction.
  • Assessing microvascular endothelial function is crucial for early diagnosis and management.
  • Non-invasive imaging techniques are needed to evaluate microvascular function.

Purpose of the Study:

  • To compare cutaneous microvascular function in healthy individuals and patients with cardiometabolic diseases.
  • To utilize laser speckle contrast imaging (LSCI) combined with acetylcholine (ACh) iontophoresis and post-occlusive reactive hyperemia (PORH).

Main Methods:

  • Cutaneous blood flow was measured using LSCI in the forearm.
  • Responses were assessed at rest, during PORH, and during iontophoresis of ACh with increasing currents (30-180 μA).

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Last Updated: May 25, 2026

A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva
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A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva

Published on: January 12, 2019

Main Results:

  • Endothelium-dependent vasodilator responses to ACh and PORH were significantly reduced in patients with cardiometabolic diseases compared to healthy subjects.
  • ACh-induced vasodilator responses were higher in young women than in young men.
  • Iontophoresis charges up to 1.5 mC showed no nonspecific effects on microvascular flux.

Conclusions:

  • Laser speckle contrast imaging (LSCI) is a promising non-invasive method for assessing systemic microvascular endothelial function.
  • Impaired microvascular function is evident in patients with cardiometabolic diseases.