Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Physiology of Taste01:24

The Physiology of Taste

The perception of a salty flavor is facilitated by sodium ions within the oral salivary fluid. Upon consumption of a salty substance, salt crystals disassemble, leading to the liberation of its constituents—Na+ and Cl- ions. These ions subsequently dissolve into the salivary fluid present in the oral cavity. The external environment of the gustatory cells experiences an elevation in Na+ concentration, thereby establishing a potent concentration gradient. This gradient propels the diffusion of...
Gustation01:43

Gustation

Gustation is a chemical sense that, along with olfaction (smell), contributes to our perception of taste. It starts with the activation of receptors by chemical compounds (tastants) dissolved in the saliva. The saliva and filiform papillae on the tongue distribute the tastants and increase their exposure to the taste receptors.
Ladder Diagrams: Complexation Equilibria01:07

Ladder Diagrams: Complexation Equilibria

Ladder diagrams are useful for evaluating equilibria involving metal-ligand complexes. The vertical scale of the ladder diagram represents the concentration of unreacted or free ligand, pL. The horizontal lines on the scale depict the log of stepwise formation constants for metal-ligand complexes and indicate the dominant species in all the regions.
The formation constant, K1, for the formation of Cd(NH3)2+ complex from cadmium and ammonia is 3.55 × 102. Log K1 (i.e. pNH3) is 2.55, and...
Taste Buds and Receptors01:20

Taste Buds and Receptors

Gustation, or the sense of taste, is intrinsically linked to the anatomical structures located on the tongue. This organ's surface, along with the entirety of the oral cavity, is adorned with stratified squamous epithelium. Evident on the tongue are elevated structures known as papillae (singular = papilla), which house the mechanisms for the transduction of gustatory stimuli. Four distinct types of papillae exist, each identified by their unique morphological attributes: the circumvallate,...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Complexation Equilibria: Overview01:23

Complexation Equilibria: Overview

Complexation reactions take place when dative or coordinate covalent bonds form between metal ions and ligands. The compounds formed in these reactions are called coordination compounds. The number of bonds formed between the metal ion and the ligands is called its coordination number. Generally, most metal ions in an aqueous solution are solvated by water molecules and thus exist as aqua complexes.
The equilibrium constant of the complexation reaction is represented as the formation constant...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: CEACAM1 impedes thyroid cancer growth but promotes invasiveness: a putative mechanism for early metastases.

Oncogene·2023
Same author

[Unexpected deposits in the upper gastrointestinal tract : When medical history matters].

Der Pathologe·2020
Same author

CEACAM1 creates a pro-angiogenic tumor microenvironment that supports tumor vessel maturation.

Oncogene·2011
Same author

CEA-Related CAMs.

Handbook of experimental pharmacology·2010
Same author

CEACAM1/VEGF cross-talk during neuroblastic tumour differentiation.

The Journal of pathology·2007
Same author

CEACAM1 impedes thyroid cancer growth but promotes invasiveness: a putative mechanism for early metastases.

Oncogene·2006

Related Experiment Video

Updated: May 25, 2026

Taste Exam: A Brief and Validated Test
07:10

Taste Exam: A Brief and Validated Test

Published on: August 17, 2018

Bitter sweetness of complexity.

A K Horst1, C Wagener

  • 1Diagnostic Center: Institute of Clinical Chemistry, University Medical Center Hamburg-Eppendorf, Martinistraβe 52, D-20246, Hamburg, ahorst@uke.uni-hamburg.de.

Topics in Current Chemistry
|February 14, 2012
PubMed
Summary

N-glycan biosynthesis complexity evolved in metazoans, impacting cellular communication and disease. The enzyme b1,6-N-acetylglucosaminyltransferase-V (GnT-V/MGAT5) is key in N-glycan synthesis and linked to cancer progression.

Area of Science:

  • Glycobiology
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Protein glycosylation pathways, particularly N-glycan biosynthesis, show increased complexity in metazoans and mammals compared to simpler organisms.
  • N-glycans regulate diverse cellular processes including adhesion, signaling, and pathogen binding, with alterations linked to developmental and inflammatory diseases, and cancer.

Purpose of the Study:

  • To review the role of N-glycans in cellular regulation and disease pathogenesis.
  • To focus on the enzyme b1,6-N-acetylglucosaminyltransferase-V (GnT-V/MGAT5) and its implications in metabolism and cancer progression.

Main Methods:

  • Review of existing literature on N-glycan biosynthesis, function, and associated pathologies.
  • Focus on the enzymatic activity and evolutionary conservation of GnT-V/MGAT5.

More Related Videos

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
13:02

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Published on: October 5, 2016

Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
07:40

Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds

Published on: February 11, 2021

Related Experiment Videos

Last Updated: May 25, 2026

Taste Exam: A Brief and Validated Test
07:10

Taste Exam: A Brief and Validated Test

Published on: August 17, 2018

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
13:02

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Published on: October 5, 2016

Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
07:40

Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds

Published on: February 11, 2021

Main Results:

  • Complex-type N-glycans, featuring β1,6-branched structures, are implicated in pathological scenarios like tumor development and metastasis.
  • GnT-V/MGAT5 is crucial for synthesizing these β1,6-branched structures in the medial Golgi.

Conclusions:

  • GnT-V/MGAT5 plays a significant role in creating specific N-glycan structures that influence cellular processes.
  • Understanding GnT-V/MGAT5's function is critical for insights into metabolic regulation and cancer progression.