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Isolation and amplification of fungal RNA for microarray analysis from host samples
Anja Lüttich1, Sascha Brunke, Bernhard Hube
1Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|February 14, 2012
Summary
Investigate fungal infections by isolating fungal RNA and analyzing gene expression. This method, focusing on Candida albicans, aids in understanding host-pathogen interactions during experimental infections.
Area of Science:
- Microbiology
- Molecular Biology
- Mycology
Background:
- Transcriptional profiling is crucial for understanding host-pathogen interactions.
- Techniques like microarrays, Serial Analysis of Gene Expression (SAGE), and RNASeq are available for fungal gene expression analysis.
- In vitro models are established for studying fungal infections.
Purpose of the Study:
- To provide methods for isolating fungal RNA from in vitro models.
- To detail protocols for investigating transcriptional changes during experimental fungal infections.
- To adapt these methods for various fungal species, with a focus on Candida albicans.
Main Methods:
- Isolation of fungal RNA from in vitro infection models.
- Application of transcriptional profiling techniques (e.g., RNASeq, microarrays).
- Adaptation of protocols for Candida albicans and other fungi.
Main Results:
- Established protocols for fungal RNA isolation.
- Demonstrated applicability to studying transcriptional dynamics in experimental infections.
- Validated methods for Candida albicans with potential for broader fungal applications.
Conclusions:
- The described methods enable effective transcriptional profiling of fungal pathogens during infection.
- These protocols are valuable for studying host-pathogen interactions in various fungal species.
- The research provides a foundation for further investigation into fungal pathogenesis and antifungal strategies.
