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Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

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Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience
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Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

Published on: November 15, 2024

The Build-a-Genome course.

Eric M Cooper1, Helöise Müller, Srinivasan Chandrasegaran

  • 1High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 14, 2012
PubMed
Summary
This summary is machine-generated.

The Build-a-Genome course offers undergraduates hands-on synthetic biology experience by contributing to constructing a synthetic yeast cell. This intensive program provides a graduate-level research immersion, fostering essential molecular biology skills and project ownership.

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Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Biotechnology Education

Background:

  • Synthetic biology is an emerging scientific field focused on designing and constructing new biological parts, devices, and systems.
  • Undergraduate education in synthetic biology is crucial for training future scientists in this rapidly advancing area.
  • Experiential learning in laboratory settings significantly enhances student understanding and skill development.

Purpose of the Study:

  • To describe the organization and curriculum of the Build-a-Genome intensive laboratory course.
  • To provide guidance for establishing similar synthetic biology courses at other institutions.
  • To highlight the benefits of integrating students into authentic research projects.

Main Methods:

  • An intensive laboratory course structure combining lectures with hands-on molecular biology techniques.
  • Student participation in an ongoing research project to create a synthetic eukaryotic cell (yeast).
  • Providing students with significant project ownership, experimental troubleshooting opportunities, and regular scientific presentations.

Main Results:

  • Students acquire fundamental molecular biology skills through practical application.
  • Undergraduates gain a graduate student-like research experience, fostering independence and problem-solving abilities.
  • The course successfully integrates students into a real-world synthetic biology endeavor.

Conclusions:

  • The Build-a-Genome course model effectively trains undergraduates in synthetic biology through authentic research.
  • Implementing similar intensive laboratory courses can enhance biotechnology education and skill development.
  • This approach offers a scalable and effective method for introducing students to cutting-edge scientific research.