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Cresyl violet: a red fluorescent Nissl stain
A Alvarez-Buylla1, C Y Ling, J R Kirn
1Rockefeller University, New York, NY 10021.
Journal of Neuroscience Methods
|August 1, 1990
Summary
Neurobiologists can now use cresyl violet as a red fluorescent Nissl stain. This simple modification is compatible with other fluorescent tracers, enhancing microscopy techniques.
Area of Science:
- Neurobiology
- Microscopy
- Histology
Background:
- Cresyl violet is a common stain for visualizing Nissl substance in neurobiology.
- Current methods utilize bright-field microscopy, limiting compatibility with other fluorescent techniques.
Purpose of the Study:
- To describe a novel method for using cresyl violet as a red fluorescent Nissl stain.
- To enhance compatibility with multicolor fluorescence microscopy.
Main Methods:
- Minor modifications to the standard bright-field cresyl violet staining procedure.
- Key modification involves diluting the cresyl violet stain.
Main Results:
- Successfully adapted cresyl violet for red fluorescence microscopy.
- The fluorescent cresyl violet stain is compatible with other fluorescent dyes like fluorescein, Fluoro-Gold, and Fast Blue.
Conclusions:
- Fluorescent red cresyl violet staining is a simple and effective technique.
- This method offers broad utility for researchers in fluorescence microscopy.