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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Related Experiment Video

Updated: May 24, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Advances in proline ligation.

Steven D Townsend¹, Zhongping Tan, Suwei Dong

¹Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.

Journal of the American Chemical Society

|February 16, 2012

Summary

This summary is machine-generated.

This study explores native chemical ligation for N-terminal proline, finding that a selenol group in an oxidized dimer improves results over a thiyl-proline derivative.

Related Experiment Videos

Last Updated: May 24, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

Published on: July 5, 2024

Area of Science:

Chemical biology
Organic synthesis
Peptide chemistry

Background:

Native chemical ligation (NCL) is a powerful method for peptide synthesis.
Incorporating proline residues, especially at the N-terminus, presents unique challenges in NCL.
Existing NCL strategies require modification for proline residues.

Purpose of the Study:

To adapt native chemical ligation strategies for peptides with an N-terminal proline residue.
To investigate and compare different chemical approaches for achieving efficient ligation at proline.

Main Methods:

Synthesis of a 3R-substituted thiyl-proline derivative for NCL.
Synthesis of a 3R-substituted selenol derivative for NCL.
Incorporation of these modified proline derivatives into peptide sequences.
Utilizing an oxidized dimer strategy for ligation.

Main Results:

The thiyl-proline derivative approach showed limited success.
The selenol-containing proline derivative, within an oxidized dimer context, yielded improved ligation outcomes.
Demonstrated feasibility of NCL with N-terminal proline using the selenol strategy.

Conclusions:

Native chemical ligation can be successfully applied to peptides with N-terminal proline residues.
A selenol-based approach offers an effective strategy for overcoming proline-related ligation challenges.
The findings expand the utility of NCL in synthesizing proline-containing peptides.