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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Flame Photometry: Lab01:16

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In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Flame Photometry: Overview01:02

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Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...

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An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue
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A radiometry protocol for UVGI fixtures using a moving-mirror type gonioradiometer.

John Zhang1, Robert Levin, Robert Angelo

  • 1Lunera Lighting, Redwood City, California, USA.

Journal of Occupational and Environmental Hygiene
|February 16, 2012
PubMed
Summary

Ultraviolet germicidal irradiation (UVGI) using UV-C light is vital for infection control. A new measurement system enables standardized data for UVGI fixture placement, improving air purification in shared spaces.

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Area of Science:

  • Infection Control Engineering
  • Photometry and Radiometry
  • Environmental Health

Background:

  • Ultraviolet germicidal irradiation (UVGI) with 254 nm UV-C is a key strategy against airborne pathogens like TB and influenza.
  • The rise of drug-resistant TB and novel viruses (SARS, H1N1) necessitates effective engineering controls in high-risk environments.
  • Uniform UV-C distribution in upper rooms is proven effective for air decontamination, but practical fixture placement is hindered by a lack of standardized measurement methods.

Purpose of the Study:

  • To establish a standard method for measuring and reporting the spatial emission distribution of commercial UVGI fixtures.
  • To develop a radiometry measurement system for obtaining 3D radiant intensity distributions of UVGI fixtures.
  • To facilitate the development of user-friendly software for optimal UVGI fixture placement, similar to interior lighting applications.

Main Methods:

  • Adapted standard photometric methods for general lighting to UVGI fixture data reporting.
  • Developed a novel radiometry measurement system featuring a Type C goniometer, mirror, radiometer, and computer control.
  • Acquired 3D spatial emission distribution data for three exemplary UVGI fixtures.

Main Results:

  • A standardized electronic file format for UVGI fixture photometric data was created.
  • The developed radiometry system achieved measurement variations within ±2.0% across repeated trials.
  • The system successfully captured 3D radiant intensity distributions for the tested UVGI fixtures.

Conclusions:

  • The developed measurement system provides essential data for standardizing UVGI fixture performance evaluation.
  • This work lays the foundation for software that simplifies the design of effective UVGI air purification systems.
  • Standardized data and placement tools will enhance the efficacy of UVGI as an infection control strategy in public spaces.