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A graphene oxide-peptide fluorescence sensor for proteolytically active prostate-specific antigen.

Tingting Feng¹, Duan Feng, Wen Shi

¹Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Molecular Biosystems

|February 16, 2012

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A new graphene oxide-peptide sensor detects active prostate-specific antigen (PSA) by measuring fluorescence changes. This simple method accurately quantifies PSA in urine samples.

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biochemistry

Background:

Prostate-specific antigen (PSA) is a key biomarker for prostate cancer detection and monitoring.
Existing PSA detection methods can be complex and time-consuming.
There is a need for simpler, more efficient PSA detection strategies.

Purpose of the Study:

To develop a novel, sensitive, and straightforward sensor for detecting proteolytically active prostate-specific antigen (PSA).
To utilize graphene oxide (GO) and a specifically designed peptide for fluorescence-based PSA detection.
To demonstrate the sensor's applicability in biological samples like urine.

Main Methods:

Assembly of a sensor using fluorescein isothiocyanate (FITC)-labeled peptide and graphene oxide (GO).

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Utilizing the fluorescence quenching effect of GO on the labeled peptide.

Monitoring the dose- and time-dependent increase in fluorescence upon PSA-mediated peptide cleavage.

Main Results:

The GO-peptide sensor exhibited fluorescence quenching, which was reversed upon PSA cleavage.
A significant increase in fluorescence intensity was observed, directly correlating with PSA concentration.
The sensor demonstrated effective PSA detection in spiked urine samples, indicating practical applicability.

Conclusions:

The developed GO-peptide sensor offers a simple and effective method for detecting proteolytically active PSA.
This approach provides a sensitive and dose-dependent fluorescent readout for PSA quantification.
The sensor's ease of assembly and application in urine samples highlight its potential for clinical diagnostics.