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Updated: May 24, 2026
Obtaining High-Quality Cryosections of Whole Rabbit Eye
Published on: November 10, 2023
[Study on gene expression profiles of rabbit retina transient IOP during LASIK]
Hai-xia Zhao1, Chun-mei Niu, Wen-ying Guan
1Department of Ophthalmology, Affiliated Hospital of Inner Mongolia Medical College, NEIMENGGU 010050, China. zhaohaixia@sohu.com
Objective:
To detect the changes of gene expression profiles of retina by way of gene chip technology, and to investigate possible molecular pathogenesis of RGCs apoptosis caused by transient high IOP.
Methods:
Twenty-two new Zealand white rabbits were randomized to experimental control and negative pressure suction 3 min groups. The rabbit were executed at different time points of post-LASIK, such as postoperative instant, 7 d, 10 d, then the total RNA of retinal tissue was extracted to detect gene expression profiles by the way of Agilent rabbit one-way gene chip.
Results:
Instant after negative pressure suction, the genes differential expressed had 704 genes. Of these, the expression of 485 genes were unregulated and 219 genes down regulated. The higher expression of apoptosis genes were CRYAA, CRYAB, TLR3 and KRT18. 7 d after negative pressure suction, the genes differential expressed had 482 genes. Of these, the expression of 178 genes were unregulated and 304 genes down regulated. The higher expression of apoptosis genes were CRYAB, IL1-BETA and IL1R1. 10 d after negative pressure suction, the genes differential expressed had 402 genes. Of these, the expression of 213 genes were unregulated and 189 genes down regulated. The higher expression of apoptosis genes were CRYAB, CRYBA3, CRYBB2, IL1-BETA and IL1R1.
Conclusion:
The gene expression had changed after negative pressure suction. The genes with higher fold change protected RGCs from apoptosis.
