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Updated: May 24, 2026
08:35
In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Published on: June 12, 2017
[X-ray induces autophagy in human mesenchymal stem cells]
Zhe Chen1, Hai Bai, Yao-zhu Pan
1Institute of Hematology, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi
|February 17, 2012
Summary
Irradiation induces autophagy in human bone marrow mesenchymal stem cells (hBMMSC), which may protect them from injury. Autophagy markers increased with radiation, suggesting a protective role within a specific dose range.
Area of Science:
- Cell Biology
- Stem Cell Research
- Radiation Biology
Background:
- Human bone marrow mesenchymal stem cells (hBMMSC) are crucial for tissue repair.
- Understanding cellular responses to irradiation is vital for radiation therapy and safety.
Purpose of the Study:
- To investigate the induction of autophagy in hBMMSC following X-ray irradiation.
- To determine the role of autophagy in protecting hBMMSC from radiation-induced damage.
Main Methods:
- Assessing apoptosis and necrosis rates using Annexin V and propidium iodide staining.
- Observing autophagy via transmission electron microscopy.
- Analyzing mRNA expression of autophagy-related genes (Beclin1, LC3) using RT-PCR.
Main Results:
- Apoptosis initially decreased then significantly increased at 10 Gy, while necrosis steadily rose with irradiation dose.
- Autophagic vacuoles were abundant in irradiated cells, with higher proportions at 8 Gy than 10 Gy.
- mRNA levels of Beclin1 and LC3 were elevated in irradiated cells, particularly at 8 Gy.
Conclusions:
- X-ray irradiation can induce autophagy in hBMMSC.
- Autophagy appears to play a protective role for hBMMSC against irradiation injury within a certain dose range.
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