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Modular deposition chamber for in situ X-ray experiments during RF and DC magnetron sputtering

Bärbel Krause1, Susan Darma, Marthe Kaufholz

  • 1ANKA, Institut für Synchrotronstrahlung, Karlsruher Institut für Technologie, Karlsruhe, Germany. baerbel.krause@kit.edu

Journal of Synchrotron Radiation
|February 18, 2012
PubMed

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...