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Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

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Related Experiment Video

Updated: May 24, 2026

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

[Generation of engineering Th17 cells and its function evaluation].

Huan-xin Zhang1, Chong Chen, Ling-yu Zeng

  • 1The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.

Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi
|February 21, 2012
PubMed
Summary
This summary is machine-generated.

This study successfully generated engineering Th17 cells from mouse naïve T cells using RORγt overexpression and cytokine polarization. These engineered cells exhibit phenotypes and functions similar to natural Th17 cells, showing potential for immunological research.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Context:

  • Th17 cells are crucial for adaptive immunity and autoimmunity.
  • Generating Th17 cells in vitro is essential for studying their roles.
  • Existing methods for Th17 cell generation have limitations.

Purpose:

  • To engineer Th17 cells from mouse CD4(+)CD25(-) naïve T cells.
  • To evaluate the phenotype and function of engineered Th17 cells.
  • To compare engineered Th17 cells with natural Th17 cells.

Summary:

  • Recombinant lentivirus expressing RORγt was used to transduce mouse naïve T cells.
  • Co-expression of RORγt with TGF-β and IL-6 significantly increased Th17 cell generation efficiency.
  • Engineered Th17 cells demonstrated cytokine profiles (IL-17A, IL-17F, IL-21) and EAE induction capacity similar to natural Th17 cells.

Impact:

  • Establishes a high-yield method for generating functional Th17 cells.
  • Provides a valuable tool for studying Th17 cell biology and related diseases.
  • Facilitates research into autoimmune disorders and immune responses.