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Updated: May 24, 2026

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

[Expression and procoagulant activity of phosphatidylserine on the normal blood cells]

Shu-chuan Liu¹, Jin Zhou, Jia-lan Shi

¹Department of Hematology, the First Affiliated Hospital of Harbin Medical University, China.

Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi

|February 21, 2012

Abstract

Objective:

To investigate the expression and procoagulant activity of phosphatidylserine (PS) on the normal peripheral blood cells of adults.

Methods:

Normal peripheral blood samples were collected from 10 healthy volunteers (5 ml from each volunteer), platelets, neutrophils, lymphocytes and erythrocytes were isolated. The expression and procoagulant activity of PS on normal blood cells were identified by flow cytometry, inhibition test with lactadherin as PS probe and coagulation anticoagulant, respectively.

Results:

There was PS expression on a few normal blood cells (9.1%, 5.4%, 3.9% and 3.2% in platelets, neutrophils, lymphocytes and erythrocytes, respectively). The PS on these normal blood cells in vitro showed significant procoagulant activity. The plasma recalcification time was shortened by 47%, 36.5%, 25% and 12.5% by platelets, neutrophils, lymphocytes and erythrocytes, respectively; the formation of factor Xa (through both intrinsic and extrinsic pathways) and thrombin was also increased by 13% - 26% by platelets, neutrophils, lymphocytes and erythrocytes, respectively.

Conclusion:

The PS on normal blood cells in vivo may play a crucial role in the coagulation cascade.

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