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A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model
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Enhanced Procoagulant Activity on Blood Cells after Acute Ischemic Stroke
Zhipeng Yao1, Lixiu Wang2, Xiaoming Wu1
1Department of Hematology, the First Hospital, Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, 150001, China.
Translational Stroke Research
|September 22, 2016
Summary
Phosphatidylserine (PS) exposure on blood cells and microparticles increases in acute ischemic stroke (AIS), promoting clotting. Targeting PS may offer a new therapeutic strategy for stroke patients.
Area of Science:
- Biochemistry
- Hematology
- Vascular Biology
Background:
- The role of phosphatidylserine (PS)-mediated procoagulant activity (PCA) in acute ischemic stroke (AIS) is not fully understood.
- Early detection of PS exposure and its impact on coagulation is crucial for understanding stroke pathophysiology.
Purpose of the Study:
- To investigate the dynamic changes in PS exposure on blood cells and microparticles (MPs) in AIS patients.
- To evaluate the correlation between PS exposure, procoagulant activity, and stroke severity.
Main Methods:
- Flow cytometry was used to quantify PS exposure on erythrocytes, platelets, and leukocytes.
- Levels of PS-exposing microparticles (MPs) and thrombin generation were measured.
- The effect of a PS antagonist (lactadherin) on coagulation was assessed.
Main Results:
- AIS patients showed significantly higher PS exposure on blood cells and MPs compared to controls.
- PS exposure correlated with shortened clotting time, increased FXa and thrombin generation.
- PS levels, particularly on platelets and MPs, correlated with stroke severity and were higher in cardioembolic stroke.
Conclusions:
- PS exposure on blood cells and MPs contributes to the hypercoagulable state in AIS.
- PS represents a potential therapeutic target for managing acute ischemic stroke.
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