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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
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Related Experiment Video

Updated: May 24, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

TALE nucleases: tailored genome engineering made easy.

Claudio Mussolino1, Toni Cathomen

  • 1Institute of Experimental Hematology, Hannover Medical School, Hannover, Germany.

Current Opinion in Biotechnology
|February 21, 2012
PubMed
Summary
This summary is machine-generated.

Transcription activator-like effector nucleases (TALENs) are powerful tools for precise genome editing. This review covers their technological background and current applications in research and therapy.

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Last Updated: May 24, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

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Published on: April 2, 2014

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

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Published on: February 2, 2016

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Designer nucleases are essential for precise genome alteration in research and therapy.
  • Transcription activator-like effector nucleases (TALENs) have emerged as a key genome editing technology.

Purpose of the Study:

  • To review the technological background of TALENs.
  • To provide an overview of the current state-of-the-art of TALENs for genome engineering.

Main Methods:

  • Literature review of TALEN technology.
  • Analysis of TALENs' activity and specificity.

Main Results:

  • TALENs are a chief technology for targeted genome editing.
  • Applications span model organisms and human stem cells.

Conclusions:

  • TALENs offer precise genome engineering capabilities.
  • Their activity and specificity are crucial for advanced applications.