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Related Experiment Video

Updated: May 24, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Experimental approaches to identify cellular G-quadruplex structures and functions.

Marco Di Antonio1, Raphaël Rodriguez, Shankar Balasubramanian

  • 1University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge CB2 1EW, UK.

Methods (San Diego, Calif.)
|February 21, 2012
PubMed
Summary

Guanine-quadruplexes (G-quadruplexes) are DNA structures impacting cellular processes. This review covers new methods for imaging and detecting G-quadruplexes in genomic DNA for therapeutic targeting.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Guanine-rich nucleic acids form non-canonical G-quadruplex secondary structures.
  • G-quadruplex formation influences critical cellular processes like transcription, translation, and telomere maintenance.
  • These structures are implicated in genomic instability, making them potential therapeutic targets.

Purpose of the Study:

  • To review and discuss recent advancements in cellular imaging of G-quadruplexes.
  • To explore experimental genomic approaches for detecting G-quadruplexes across genomic DNA.
  • To highlight the application of engineered molecules and natural proteins for G-quadruplex detection.

Main Methods:

  • Fluorescence imaging techniques for G-quadruplex visualization.

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

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In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
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In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Related Experiment Videos

Last Updated: May 24, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

  • Genomic approaches including pull-down assays, ChIP-Seq, and ChIP-chip.
  • Utilizing engineered small molecules and natural proteins as probes.

    • Main Results:

    • Recent methods enable sensitive and specific detection of G-quadruplexes within cellular DNA.
    • Various experimental approaches allow for the mapping of G-quadruplexes throughout the genome.
    • Engineered molecules and proteins serve as effective tools for G-quadruplex detection and imaging.

    Conclusions:

    • Developing tools for G-quadruplex detection is crucial for understanding their biological roles.
    • These methods offer potential for investigating G-quadruplex involvement in disease.
    • Advancements in G-quadruplex imaging and detection pave the way for novel therapeutic strategies.