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Published on: November 12, 2017
Protein kinase activity profiling of postmortem human brain tissue.
Jeroen J M Hoozemans1, Riet Hilhorst, Rob Ruijtenbeek
1Department of Pathology, Neuroscience Campus Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
Neuro-Degenerative Diseases
|February 21, 2012
Summary
This study used kinome profiling to identify activated protein kinases and signaling pathways in Alzheimer's disease (AD) brain tissue. Findings reveal potential new targets for AD biomarkers and therapies.
Area of Science:
- Neuroscience
- Biochemistry
- Molecular Biology
Background:
- Alzheimer's disease (AD) pathogenesis involves complex signal transduction pathways.
- Identifying key protein kinases in AD is crucial for developing targeted therapies and biomarkers.
Purpose of the Study:
- To identify protein kinases and signaling pathways activated in Alzheimer's disease (AD) pathology.
- To explore novel therapeutic targets and diagnostic markers for AD.
Main Methods:
- Utilized microarray-based kinome profiling on postmortem brain tissue from AD and control cases.
- Employed a peptide array system to assess global serine/threonine kinase activity.
- Quantified peptide phosphorylation using fluorescently labeled antibodies.
Main Results:
- Detected ATP-dependent peptide phosphorylation in both AD and control brain lysates.
- Identified differentially phosphorylated peptides in AD cases compared to controls.
- Revealed distinct kinase activity profiles associated with Alzheimer's disease.
Conclusions:
- Protein kinase activity profiling is a viable method for discovering novel kinases in AD.
- This approach can elucidate new signaling pathways implicated in Alzheimer's disease pathology.
- Findings pave the way for developing innovative AD biomarkers and therapeutic strategies.
