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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 24, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

A highly sensitive electrochemical assay for microRNA expression profiling.

Zhiqiang Gao1

  • 1Department of Chemistry, National University of Singapore, Singapore 117543. chmgaoz@nus.edu.sg

The Analyst
|February 21, 2012
PubMed
Summary

This study introduces a sensitive electrochemical assay for microRNA (miRNA) profiling without ligation or PCR. The novel method achieves a low detection limit for accurate miRNA expression analysis in cancer cells.

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

  • Electrochemistry
  • Biotechnology
  • Molecular Biology

Background:

  • MicroRNA (miRNA) expression profiling is crucial for understanding cellular processes and disease.
  • Existing methods for miRNA detection often involve complex procedures like ligation and polymerase chain reaction (PCR).
  • There is a need for simpler, more sensitive, and efficient miRNA detection techniques.

Purpose of the Study:

  • To develop a simple, highly sensitive, ligation-free, and PCR-free electrochemical assay for microRNA expression profiling.
  • To utilize an electrocatalytic reaction involving glucose oxidase (GOx) and glucose to enhance assay sensitivity.
  • To demonstrate the assay's capability in profiling specific miRNA expressions in biological samples.

Main Methods:

  • Fabrication of an electrode modified with stem-looped capture probes (CPs) and detection probes (DPs).
  • Utilizing an electrocatalytic amplification strategy with electrochemically activated glucose oxidase (GOx) and glucose.
  • Hybridization-triggered conformational change of the stem-looped CP to expose the DP binding site.
  • Electrochemical detection of miRNA through the catalytic activity of GOx on glucose.

Main Results:

  • Achieved a low detection limit of 4.0 fM for miRNA.
  • Demonstrated a linear calibration curve for miRNA detection up to 10 pM under optimal conditions.
  • Successfully applied the assay to profile human let-7 miRNA expression in cultured cancer cells.

Conclusions:

  • The developed electrochemical assay provides a simple and highly sensitive platform for miRNA expression profiling.
  • The assay eliminates the need for ligation and PCR, streamlining the detection process.
  • This method holds potential for accurate miRNA analysis in biological and clinical applications, including cancer research.