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Updated: May 24, 2026

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
Published on: March 11, 2020
Glutamatergic neurons induce expression of functional glutamatergic synapses in primary myotubes
Michele Ettorre1, Erika Lorenzetto, Claudia Laperchia
1Department of Neurological, Neuropsychological, Morphological and Movement Sciences, Section of Physiology and Psychology, University of Verona, Verona, Italy; National Institute of Neuroscience, Brescia and Verona, Italy; Center for Biomedical Computing, University of Verona, Verona, Italy.
Background:
The functioning of the nervous system depends upon the specificity of its synaptic contacts. The mechanisms triggering the expression of the appropriate receptors on postsynaptic membrane and the role of the presynaptic partner in the differentiation of postsynaptic structures are little known.
Methods And Findings:
To address these questions we cocultured murine primary muscle cells with several glutamatergic neurons, either cortical, cerebellar or hippocampal. Immunofluorescence and electrophysiology analyses revealed that functional excitatory synaptic contacts were formed between glutamatergic neurons and muscle cells. Moreover, immunoprecipitation and immunofluorescence experiments showed that typical anchoring proteins of central excitatory synapses coimmunoprecipitate and colocalize with rapsyn, the acetylcholine receptor anchoring protein at the neuromuscular junction.
Conclusions:
These results support an important role of the presynaptic partner in the induction and differentiation of the postsynaptic structures.
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