Related Experiment Video
Updated: May 24, 2026

Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Published on: February 22, 2020
Interactions of alginate-producing and -deficient Pseudomonas aeruginosa with zwitterionic polymers
Chun-Jen Huang1, Luo Mi, Shaoyi Jiang
1Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Abstract:
In this work, we report a study of the interactions between bacteria and zwitterionic polymers using a long-range surface plasmon resonance (LR-SPR) sensor. The LR-SPR with an extended probing field allows one to perform in-situ monitoring of bacterial adhesion and to investigate bacteria-surface interactions under various conditions. Alginate-producing and -deficient Pseudomonas aeruginosa were used in order to study the role of capsular exopolysaccharide in bacterial adhesion onto a surface. Polycarboxybetaine polymers with one and two carbons in spaces between two oppositely charged moieties on polymer side chains (pCBAA-1 and pCBAA-2) were prepared in order to unveil the effects of material properties on surface resistance to bacterial adhesion. In addition, environmental factors (e.g., divalent cations) were investigated. This work demonstrates the role of polysaccharides beyond proteins in bacteria adhesion and the effective resistance of pCBAA-1 against bacterial adhesion.
More Related Videos
08:57Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
Published on: March 10, 2014
09:27Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Related Concept Videos
Gene Regulation in Microbial Communities: Quorum Sensing
Biofilms