Smartphone-Assisted Digital Image-Based Optical Biosensor Array for Quantification of Interleukin-8 Using...

We developed a smartphone-assisted digital image-based optical biosensor array using a planar glass slide with sensor spots in a 2 × 5 array format for point-of-care multiplex detection of biomarkers. The detection is based on the integration of the capture antibody (Ab^C)-functionalized sensor array with a detection antibody-conjugated gold nanoparticle bioconjugate (AuNP@Ab^D) in the presence of interleukin-8 (IL8) to form a sandwich-type AuNP@Ab^D-IL8-Ab^C nanocomplex on the sensing spot surface. Thus, the colorimetric detection method can be applied to the quantitative analysis of IL8, a clinically relevant pro-inflammatory and pro-angiogenic biomarker. The sensing strategy utilizes digital image-based analysis via ImageJ software (V 1.54 g; Java 1.8.0_345 [64 - bit], Windows 8) to quantify the colorimetric signals generated by the light absorbance of surface-bound gold nanoparticles in response to an IL8 droplet sample of merely 8 μL on the planar glass surface, achieving a low detection limit of 0.23 pg/mL (27 fM) and good reproducibility with a coefficient of variation of 0.95%. Validation using IL8-spiked serum at concentrations of 1 × 10^-9 M and 1 × 10^-10 M showed minimal matrix effects with a detection accuracy of 99.5% and 106.1%, respectively. Hence, this low-cost portable digital image-based plasmonic nanoparticle-linked immunosorbent assay serves as an alternative to traditional enzyme-linked immunosorbent assays.