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Published on: January 16, 2017
Isolation of NEDDylated proteins in human cells
Orsolya Leidecker1, Dimitris P Xirodimas
1Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, Scotland, UK.
Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
Abstract:
Identification of substrates for post-translational modification with ubiquitin and ubiquitin-like molecules is a key in our understanding of the role of this modification in the regulation of biological processes. The ubiquitin-like molecule NEDD8 plays a vital role in cell growth, viability, and development. In this -chapter, we describe a method for efficient isolation and detection of NEDDylated proteins in human cells.
