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Comparative Strategies for Ubiquitination Detection in Mammalian Cell Lysates Using SMAD2/SMURF2 as a Model
Published on: April 17, 2026
Using linkage-specific monoclonal antibodies to analyze cellular ubiquitylation
Kim Newton1, Marissa L Matsumoto, Ronald E Ferrando
1Physiological Chemistry Department, Genentech, Inc., South San Francisco, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
Abstract:
Antibodies that specifically recognize polyubiquitin chains containing ubiquitins linked at a particular lysine residue are powerful tools for interrogating endogenous protein modifications. Here, we describe protocols for revealing K11-, K48-, and K63-linked polyubiquitin chains by western blotting, immunoprecipitation, or immunostaining.
