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Related Experiment Video

Updated: May 24, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Raising polyclonal antibodies using nitrocellulose-bound antigen.

M Diano¹, A L Bivic, M Hirn

¹Biohgie de la Differenciation Cellulaire, Faculte des Sciences de Luminy, Marseilles, France.

Methods in Molecular Biology (Clifton, N.J.)

|February 22, 2012

Summary

This summary is machine-generated.

Obtaining specific antibodies against low-abundance or insoluble proteins is challenging due to purification difficulties. This study addresses the limitations in antibody production for minor and difficult-to-purify protein targets.

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Last Updated: May 24, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

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Published on: January 2, 2017

Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

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Published on: April 6, 2019

Area of Science:

Biochemistry
Immunology
Protein Chemistry

Background:

Antibody production is crucial for detecting and studying proteins.
Minor proteins and insoluble proteins are difficult to purify for antibody generation.
Classical purification methods yield insufficient quantity and purity for antibody development.

Purpose of the Study:

To overcome challenges in obtaining specific antibodies against minor or insoluble proteins.
To improve antibody generation strategies for difficult protein targets.

Main Methods:

The abstract does not specify methods.

Main Results:

The abstract does not specify results.

Conclusions:

The abstract does not specify conclusions.