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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Related Experiment Video

Updated: May 24, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Epitope mapping.

S E Mole1

  • 1CRC Human Cancer Genetics Research Group, Department of Pathology, University of Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed
Summary
This summary is machine-generated.

Monoclonal antibodies (MAbs) are precise immunological tools that bind to specific epitopes on proteins. Epitope mapping is the process used to identify these exact binding sites, enhancing antibody research.

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Last Updated: May 24, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
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Published on: March 24, 2017

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
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Published on: March 25, 2014

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
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Area of Science:

  • Immunology
  • Biochemistry
  • Molecular Biology

Background:

  • Monoclonal antibodies (MAbs) are highly specific proteins used in various biological and medical applications.
  • Their specificity arises from binding to unique regions on target molecules, known as epitopes.
  • Understanding these binding sites is crucial for optimizing MAb utility.

Purpose of the Study:

  • To define the process and significance of epitope mapping for monoclonal antibodies.
  • To highlight the importance of precisely identifying MAb binding sites.

Main Methods:

  • Epitope mapping involves techniques to determine the exact location of antibody binding on an antigen.
  • These methods can include peptide scanning, X-ray crystallography, and mutagenesis studies.

Main Results:

  • Successful epitope mapping precisely identifies the amino acid or conformational residues that constitute the MAb binding site.
  • This detailed information confirms the specificity of the monoclonal antibody.

Conclusions:

  • Epitope mapping is an essential procedure for characterizing monoclonal antibodies.
  • Knowledge of epitope-antigen interactions advances the development and application of MAbs in research and diagnostics.