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Electron microscopic silver enhancement for double labeling with antibodies raised in the same species

K Bienz1, D Egger

  • 1Institute for Medical Microbiology, University of Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed

In immunoelectron microscopy (IEM), simultaneous labeling of two or more antigens on the same section is desirable for many applications. If the antibodies (Ab) to be used are raised in the same species, as is usually the case with monoclonal antibodies (MAb), the difficulty arises that the labeled secondary, antispecies Ab used in the first labeling step traps the primary Ab directed against the second antigen, thus leading to a nonspecific signal for the second antigen.

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